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a kinase anchoring protein 4  (Proteintech)


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    Proteintech a kinase anchoring protein 4
    A Kinase Anchoring Protein 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+kinase+anchoring+protein+4/AKAP4+Antibody/pm41549443-69-16-23
    Average 93 stars, based on 20 article reviews
    a kinase anchoring protein 4 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Novel homozygous or compound heterozygous DNAH17 variants lead to male infertility characterized by multiple morphological abnormalities of the sperm flagella and asthenoteratozoospermia in humans.
    Article Snippet: IF staining of sperm was conducted as previously described.4 Briefly, sperm samples were smeared onto slides, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 (SigmaAldrich, Darmstadt, Germany), and blocked with 3% bovine serum albumin (BSA). .. Primary antibodies against DNAH17 (1:100; 24488- 1-AP; Proteintech, Wuhan, China), β-tubulin (1:500; GB15140-100; Servicebio, Wuhan, China), A-kinase anchoring protein 4 (AKAP4; 1:100; 24986-1-AP; Proteintech), sperm-associated antigen 6 (SPAG6; 1:100; 12462-1-AP; Proteintech), radial spoke head component 9 (RSPH9; 1:100; 23253-1-AP; Proteintech), cilia- and flagella- associated protein 70 (CFAP70; 1:100; 31344-1-AP; Proteintech), dynein axonemal light intermediate chain 1 (DNALI1; 1:100; 17601-1-AP; Proteintech), translocase of outer mitochondrial membrane 20 (TOM20; 1:100; 11802-1-AP; Proteintech), intraflagellar transport 20 (IFT20; 1:100; 20536-1-AP; Proteintech), and centrosomal protein 135 (CEP135; 1:100; 24428-1-AP; Proteintech) were incubated at 4°C overnight. .. Secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488, 1:500, GB25303, Servicebio; and goat anti-mouse IgG Alexa Fluor 594, 1:500, GB28303, Servicebio) were used, and 4’,6-diamidino-2-phenylindole (DAPI) was applied to label the nuclei.

    Incubation:

    Article Title: Novel homozygous or compound heterozygous DNAH17 variants lead to male infertility characterized by multiple morphological abnormalities of the sperm flagella and asthenoteratozoospermia in humans.
    Article Snippet: IF staining of sperm was conducted as previously described.4 Briefly, sperm samples were smeared onto slides, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 (SigmaAldrich, Darmstadt, Germany), and blocked with 3% bovine serum albumin (BSA). .. Primary antibodies against DNAH17 (1:100; 24488- 1-AP; Proteintech, Wuhan, China), β-tubulin (1:500; GB15140-100; Servicebio, Wuhan, China), A-kinase anchoring protein 4 (AKAP4; 1:100; 24986-1-AP; Proteintech), sperm-associated antigen 6 (SPAG6; 1:100; 12462-1-AP; Proteintech), radial spoke head component 9 (RSPH9; 1:100; 23253-1-AP; Proteintech), cilia- and flagella- associated protein 70 (CFAP70; 1:100; 31344-1-AP; Proteintech), dynein axonemal light intermediate chain 1 (DNALI1; 1:100; 17601-1-AP; Proteintech), translocase of outer mitochondrial membrane 20 (TOM20; 1:100; 11802-1-AP; Proteintech), intraflagellar transport 20 (IFT20; 1:100; 20536-1-AP; Proteintech), and centrosomal protein 135 (CEP135; 1:100; 24428-1-AP; Proteintech) were incubated at 4°C overnight. .. Secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488, 1:500, GB25303, Servicebio; and goat anti-mouse IgG Alexa Fluor 594, 1:500, GB28303, Servicebio) were used, and 4’,6-diamidino-2-phenylindole (DAPI) was applied to label the nuclei.



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